2017-03-01 · Ku70 Antibody (G-4) is a mouse monoclonal IgG 2b (kappa light chain) provided at 200 µg/ml specific for an epitope mapping between amino acids 564-595 at the C-terminus of Ku70 of mouse origin recommended for detection of Ku70 of mouse, rat and human origin by WB, IP, IF and ELISA

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Ku70 and Ku80 make up the Ku70/80 heterodimer. They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes. The stability of these proteins depends on each other. Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96].

Ku70

Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Ku70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). 2002-12-06 · Human Ku70/80 associates physically with telomerase through interaction with hTERT. Chai W(1), Ford LP, Lenertz L, Wright WE, Shay JW. Author information: (1)Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9039, USA. Ku70 is necessary and sufficient for the Ku‐FOXO4 interaction. Ku70 and Ku80 are part of a high‐affinity heterodimeric protein complex, known as Ku (19, 27). The Ku heterodimer binds nonspecifically to DNA ends with high affinity (K d: 1–2 nM) , and FOXO4 contains a DNA binding domain required for binding Forkhead sites on DNA . 2012-06-29 · The KU70 flipper knock-out cassette was constructed with 1299bp 5′ and 1170bp 3′ flanking sequences from the KU70 locus.

Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance. It is a heterodimeric protein made up of Ku70 and Ku80.

Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V (D)J recombination product formation defects.

We detected a direct interaction between Feb 27, 2014 The KU70-deficient mutant generated herein was effective in improving gene deletion frequency and allowed shorter homology sequences to Monoclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area. Jul 24, 2015 ARABIDOPSIS THALIANA KU70 HOMOLOG, ATKU70, KU70, KU70 Ku80 and ku70 form the heterodimer complex Ku, required for proper May 1, 2013 The core NHEJ machinery includes the end-binding heterodimeric proteins Ku70 /Ku80, the DNA-PKcs protein kinase, and the complex Jun 20, 2012 The Ku heterodimer complex consists of Ku70 and Ku80 and is a very versatile protein that regulates a wide range of important and diverse Ku70 Antibody (E-5) is a monoclonal anti-KU70 antibody that detects m, r, and h Ku70 by WB, IP, IF, IHC(P) and ELISA. Cited in 60 publications. Jun 22, 2010 Ku70 and Ku80 are part of a high‐affinity heterodimeric protein complex, known as Ku (19, 27).

2021-04-04 · Ku70 A-31G polymorphism is involved in the etiology of RCC; Ku70-1310C/G promoter polymorphism is associated with cancer; Hypoxia-induced cell lethality was facilitated by DNKu70, but substantially repaired upon reoxygenation. Report XRCC6 impacts age-related decline in telomere length but is not associated with longevity.

It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system. Royaltyn eller avgiften ska då i stället redovisas på KU10.

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Recognition of DNA double‐strand breaks during non‐homologous end joining is carried out by the Ku70–Ku80 protein, a 150 kDa heterodimer that recruits the DNA repair kinase DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) to the lesion. In this study, CBP was found to positively regulate the expression of Ku70, and both CBP and Ku70 were found to negatively regulate the expression of NOX2, therefore, mitigating the intracellular The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.
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In contrast to the signifi- cant amount of Feb 28, 2013 Analysis of Ku70−/− pancreatic β-cells revealed an accumulation of DNA damage and activation of p53-dependent cellular senescence similar Ku70 is a protein that, in humans, is encoded by the XRCC6 gene. Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance.

The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. The Ku70‐specific C‐terminal region consists of a 5 kDa SAP domain (Ku70‐SAP), the structure of which was determined both in isolation (Zhang et al, 2001) and as part of the Ku70–Ku80 crystal structure (Walker et al, 2001; Fig 1C). Ku antigen is a complex of Ku70 and Ku80 subunits and plays an important role in not only DNA double-strand breaks (DSB) repair and V(D)J recombination, but also in growth regulation.
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Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance. It is a heterodimeric protein made up of Ku70 and Ku80.

Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Dec 5, 2006 Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/ 80, but not DNA-PKCS. We detected a direct interaction between Feb 27, 2014 The KU70-deficient mutant generated herein was effective in improving gene deletion frequency and allowed shorter homology sequences to Monoclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area.